Carrizo Plain National Conservation Area Act of 2000

Requires the Secretary, in cooperation with the Director, the California Department of Fish and Game, affected landowners, and The Nature Conservancy, to review and make any necessary revisions to the existing management plan.

Establishes in the Treasury a separate Carrizo Plain National Conservation Area Management Fund.

Authorizes the Secretary to acquire nongovernment, privately owned lands and interests within the conservation area by donation, exchange, or purchase with the owner's consent.

Withdraws all Federal lands within the conservation area, including all subsequently acquired lands or interests, from all forms of entry, appropriation, or disposal under the public land laws and from location, entry, and patent under Federal mining laws. Allows mineral development in the conservation area only to the extent consistent with the management plan.

Authorizes the Secretary to enter into cooperative agreements or shared management arrangements with any person for management, interpretation, and research of the conservation area's resources.

Directs the Secretary to ensure nonexclusive access to and use of the public lands in the conservation area by Native Americans for traditional cultural and religious purposes consistent with the American Indian Religious Freedom Act. Permits the Secretary from time to time temporarily to specific areas in such lands close to general public use in order to protect the privacy of Native American religious activities.